Volume 10, Issue 3: 131-139; May 27, 2020  
ISSN 2228-7701  
EPIDEMIOLOGY, DIAGNOSIS AND PUBLIC HEALTH  
IMPORTANCE OF TRICHINELLOSIS  
Muluken Yayeh, Gemechis Yadesa, Mareye Erara, Shumye Fantahun, Alemseged Gebru and Mastewal Birhan  
University of Gondar, Collage of Veterinary Medicine and Animal Sciences, Ethiopia  
Email: yayehmulu@gmail.com  
Supporting Information  
ABSTRACT: Trichinellosis is a parasitic zoonosis caused by Trichinella following ingestion of raw or under cooked  
meat containing Trichinella larvae. Nematode worms of the genus Trichinella are one of the most prevalent  
zoonotic pathogens in the world. The parasite infects domestic and wild animals and has a worldwide  
distribution. The life cycle of the parasite consists of a domestic cycle in mainly pigs and a sylvatic cycle in a  
wider range of animals such as bears and wild boar. Humans become infected after eating raw or undercooked  
meat from domestic pigs, horses or game containing Trichinella larvae. There are twelve genotypes within the  
genus Trichinella, eight of which have been designated as species from which T.spiralis is the most pathogenic  
one. Host animals ingesting even high numbers of Trichinella larvae from infectious meat will not develop  
clinical symptoms. In humans, the clinical picture is usually illustrated by an intestinal stage within the first or  
second week after infection and later muscular stage with periorbital oedema, myalgia or muscle weakness as  
the major symptoms. The severity of the clinical course depends firstly on parasitic factors, such as the species  
implicated and the number of infective larvae ingested, and secondly on host factors, such as sex, age, and  
immune status. In practice, treatment with anthelmintics and immunosuppressive drugs is used only with  
human patients, not with animals. Trichinella infection in humans is strongly associated with the consumption of  
raw or undercooked meat; thus, cultural factors such as traditional dishes based on raw or undercooked meat or  
meat-derived products.  
Keyword: Human, Parasite, Pig, raw meat, Trichinellosis; Zoonosis  
INTRODUCTION  
Parasitic zoonosis includes both helminthic and protozoan infections. Amongst one thousand five hundred known  
infectious agents for human being, 287 are helminths (Chomel et al., 2008). Helminths are complex eukaryotic  
organisms with large genomes and complex multistage life cycles that involve numerous hosts (Hewitson et al., 2009).  
Nematode worms of the genus Trichinella are one of the most widespread zoonotic pathogens in the world. Infection by  
Trichinella species has been identified in domestic and/or wild animals of all continents, with the exception of Antarctica,  
where there is no record of the parasite (Murrell, 2006).  
Clinical signs of trichinellosis are not generally recognised in animals, and its main importance is as a zoonosis.  
Trichinosis is a food-borne zoonotic disease caused by Trichinella species. Trichinellosis in humans is caused by eating raw  
or undercooked meat from Trichinella-infected food animals or game (Gajadhar et al., 2006). Until recently, all Trichinella  
infections occurring in animals and humans were attributed to Trichinella spirals. Today, eight species (T.spiralis, T.nativa,  
T.britovi, T.pseudospiralis, T.murrelli, T.nelsoni, T.papuae, and T.zimbabwensis and three genotypes (Trichinella T6, T8, T9)  
within two classes (encapsulated and non-encapsulated) are documented in this genus (Zarlenga et al., 2006).  
It is a tissue-dwelling nematode acquired by the ingestion of raw or insufficiently cooked meat-products containing  
encapsulated larvae (La Rosa et al., 2000). The most important source of human infection worldwide is the domestic pig.  
In Europe, meats of horses and wild boars have played a significant role during outbreaks within the past three decades.  
Infection of humans occurs with the ingestion of Trichinella larvae that are encysted in muscle tissue of meat from  
domestic or wild animals (Bruschi et al., 2007).  
Trichinella infection in the human host can be divided into two stages: an intestinal (or enteral) phase and a  
muscular (or parenteral or systemic) phase. Low-intensity infection can remain asymptomatic, but parasite burdens  
greater than a few hundred larvae can initially cause gastroenteritis associated with diarrhoea and abdominal pain  
approximately 2 days post infection (intestinal acute phase of disease). Subsequently, migrating larvae and their  
metabolites provoke an immediate reaction, with an inflammatory and allergic response, pyrexia, eyelid or facial oedema,  
and eosinophilia are the most prominent manifestations, occasionally complicated by myocarditis, thromboembolic  
disease, and encephalitis. Months or even years at the acute stage, chronic trichinellosis may yield persistent fornication,  
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numbness, and excessive sweating as well as impaired muscle strength and conjunctivitis, which may continue up to 10  
years post infection (Zarlenga et al., 2013).  
The diagnosis of trichinellosis is based on history of consumption of potentially contaminated meat, the presence of  
compatible signs and symptoms, and identification of Trichinella larvae in biopsy muscle tissue or specific antibody in  
serum. These diagnostic methods in human host can be categorized two as direct and indirect. Under direct there are  
direct muscle biopsy while under indirect such as serology and molecular technique (Oivanen, 2005). Muscle biopsies are  
rarely performed, but they allow for the molecular identification of the Trichinella species or genotype, which is not  
possible with antibody testing (Oivanen, 2005).  
Prompt treatment with anti-parasitic drugs can help prevent the development of trichinellosis by killing the adult  
worms and so preventing further release of larvae. Once the larvae have become established in skeletal muscle cells,  
treatment may not completely eliminate the infection and associated symptoms (Sun, 2015). Therefore, the  
administration of effective anthelmintic drugs at the stage of intestinal invasion or in the acute phase is critical for  
successful therapy. In addition, because of the predominantly zoonotic importance of infection, the main efforts in many  
countries have focused on the control or elimination of Trichinella from the food chain (Gottstein, 2009).  
The increase in the report of Trichinellosis has been observed many eastern European countries, in Africa and Asia  
(Blaga et al., 2007; Azim et al., 2008). Human population growth and socioeconomic changes might have played a  
fundamental role in the disease emergence and spread in recent years (Macpherson, 2005). The increase in human  
population density, ecological change, and subsequent increased contact between humans and wild animals necessitates  
the importance having an update on potentially emerging diseases like trichnellosis. Therefore, the objective of this paper  
is to review the epidemiology and public health importance of trichinellosis.  
Taxonomy and Morphology of the Parasites  
Taxonomy and Aetiology  
The taxonomy of the genus Trichinella has been presented with slightly varying details According to the traditional  
classification, the genus belongs to the phylum Nematode, roundworms, class Adenophorea, order Trichinellida, and  
superfamily Trichinelloidea (Oivanen, 2005). The taxonomy has recently been challenged. On the basis of results from  
ribosomal deoxyribonucleic acid (DNA) sequences, the present higher-level classification of Nematode will need change in  
to two classes, Secernentea and Adenophorea (Oivanen, 2005). Within the genus Trichinella there are twelve genotypes  
have been identified, eight of which have been designated as species (Gajadhar et al., 2006; Murrell et al., 2000; Pozio  
and Zarlenga, 2005). Trichinella spiralis was recognized in London in 1835s. The parasite being detected in an autopsy of  
an Italian male corpse (Oivanen, 2005).  
Trichinella spiralis (T1) is distributed in temperate regions world-wide and is commonly associated with domestic  
pigs. It is highly infective for domestic and sylvatic swine, mice and rats, but it can also be detected in other mammalian,  
carnivores and horses (Pozio and Zarlenga, 2005). This species is also the most important etiological agent to cause  
disease in humans (Pozio, 2006). Trichinella native is the species that are very widespread in arctic and subarctic areas of  
the northern hemisphere (Pozio, 2000). Trichinella britovi species differs from T. spiralis with weak infectivity for rats,  
moderate resistance to freezing, moderate infectivity for swine, slow nurse cell development and low in vitro production of  
NBL (Malakauskas and Kapel, 2003). Trichinella nelson has occasionally been detected in pigs (Suidae) and humans,  
although it has very low infectivity for pigs and rats. The infectivity for humans has not been long-established (Pozio,  
2001).  
Trichinella murrelli this species has very low reproductive capacity in pigs and rats, low NBL production in vitro, slow  
nurse cell development, and low resistance to freezing (Malakauskas and Kapel, 2003). Trichinella pseudospiralis strains  
three genotypic isolates were identified by multiplex polymerase chain reaction from different parts of the world (PCR)  
test (Zerlenga et al., 1999; La Rosa et al., 2001). Trichinella papuae are where Muscle larvae are non-encapsulated and  
lack freezing tolerance but can survive in +5oC storage for four weeks (Webster et al., 2002). Trichinella zimbabwensis is  
the first Trichinella strain isolated in reptiles in nature. In the laboratory, it can also infect rats, mice, pigs, baboons (Papio  
sp.), turtles, pythons, varans, and caimans. Its muscle larvae are non-encapsulated. It is not infective for birds, nor can it  
resist freezing (Pozio et al., 2004).  
Morphology of the Parasite  
Trichinella worms are the smallest nematode parasite of humans, they are the largest intracellular parasite and  
have been described as “the worm that would be virus” (Foreyt, 2013). The morphology of the parasite’s oesophagus is  
characteristic of the Trichinellidae family, and it occupies approximately one-third of the body length and is surrounded by  
large cells. Adult males are 1.4 to 1.6 mm in length and do not have spicules, but a pair of lateral flaps is found on each  
side of the cloacal opening and two pairs of papillae are between them. Females’ are 3 to 4 mm in length, and the vulva  
opens in the middle of the oesophageal region (Foreyt, 2013). Adult the length of T. spiralis NBL is 80-120 μm and the  
diameter 5-6 μm. The larvae do not increase in size until they enter the muscle cells. The larvae begin to grow in their  
nurse cells, reaching a length of 900-1280 μm and a diameter of 35-40 μm by 30days p.i. Trichinella adult females are a  
little longer and thicker than the males. Their length and diameter are 2460-3390 μm and 35-70 μm, respectively, while  
the resulting figures for males are 1040-1300 μm and 29-32 μm (Oivanen, 2005).  
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Figure 1- Morphology of parasites (Foreyt, 2013)  
The life cycle of the parasites  
The basic life cycle of Trichinella has been recognized since the middle of the 19th century. This genus is unique  
among parasitic nematodes in that all stages of the life cycle occur within a single host. In nature, the cycle is repeated  
when another host animal ingests the flesh of another host containing viable muscle-stage larvae (Oivanen, 2005). The  
generalized life cycle of Trichinella is described in figure 2. Enteral phase; 1: muscle tissues are digested in the stomach  
and larvae are released; 2: larvae penetrate the intestinal mucosa of the small intestine, reach the adult stage within 48 h  
post infection, male and female mate; 3: female worm releases new born larvae in the lymphatic vessels (from the fifth  
day post infection onwards; the length of New born production, from one to several weeks, is under the influence of the  
host immunity). Parenteral phase; 4: the new born larva reach the striated muscle and actively penetrate in the muscle  
cell; 5: the larva grows to the infective stage in the nurse ceil (the former muscle cell); 6: after a period of time (weeks,  
months or years) a calcification process occurs (Pozio and Murrell, 2006).  
Figure 2- Trichinella sp. life cycle (Pozio and Murrell, 2006). A: main sources of Trichinella spp. infections for humans; B:  
Trichinella spp. cycle in the host body.  
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Epidemiology  
Geographic distribution  
Trichinella species are present throughout most of the world in over 150 different hosts (Dick et al., 2001). In  
addition to T. spiralis, 7 other species in 4 genotypes, all of which are more commonly found in wild animals than in  
domestic pigs . T. spirals is cosmopolitan, this species is also the most important etiological agent to cause disease in  
humans (Pozio et al., 2006). In the domestic cycle, pork scraps from T. spiralis-infected pigs are the main source of  
infection for synanthropic animals (e.g., rats, horses, stray cats, and dogs). Conversely to the domestic cycle, the sylvatic  
cycle of T. spiralis includes a broad range of wild carnivores, which may, however, become the origin of a life cycle  
beginning into a domestic host population (Dick, 2001). Trichinella native is found in Arctic and subarctic areas of  
America, Asia, Europe. Trichinella genotype T6 is also found in Canada, Alaska, Rocky Mountains, and Appalachian  
Mountains in the United States (Pozio, 2001). Trichinella britovi is found in the temperate areas of Europe and Asia,  
Northern and Western Africa, Trichinella T8 is found in South Africa and Namibia. Trichinella murrelli is found in the United  
States and Southern Canada, Trichinella genotype T9 Japan, T. nelsoni Eastern-Southern Africa, and Trichinella genotype  
T12 is found in Argentina, and all the above are encapsulated while the below are non-capsulated. Those are T.  
pseudospiralis which is cosmopolitan in Palearctic, Nearctic, Ocean land (Pozio, 2001) Cosmopolitan , T. papuae is found  
in the Papua New Guinea, Thailand and T. zimbabwensis which is found in Zimbabwe, Mozambique, Ethiopia, South Africa  
Host range  
The epidemiology and systematics (i.e., the study of the diversification) of this zoonosis are now recognized to  
involve in addition to T. spiralis, 7 other species in 4 genotypes, all of which are more commonly found in wild animals  
than in domestic pigs (Pozio, 2009). T. spiralis is found in the Domestic and sylvatic mammal, while Trichinella T8, T.  
murrelli, Trichinela genotype T9. Trichinella native and Trichinella genotype T6 is found in the sylvatic carnivores. Another  
species, T. britovi is found in the Sylvatic mammals and seldom domestic pigs, while T. nelson in the Sylvatic mammals.  
Trichinella genotype T12, T. Pseudo spiralis Sylvatic mammals and birds, domestic pigs, T. papuae Wild pigs, salt water  
crocodiles and T.zimbabwensis which is found in the Nile crocodiles, monitor lizards (Gottstein, 2009).  
Main source of infection in human  
Domestic pigs and wild boars were the major sources of Trichinella spp. infection for humans, but in recent years  
new infection sources, particularly from exotic hosts, have emerged (Boireau, 2000). The main source of infection in  
human, T. spiralis is found in the Domestic and sylvatic swine horses, while T. native is found in Bears, walruses. Others  
like Trichinella genotype T6 is found in Carnivores T. britovi is found in the Wild boars, domestic pigs horses, foxes, jackals  
T. zimbabwensis, Trichinella genotype T12, Trichinella genotyp T8 and Trichinella genotype T9. Trichinella murrelli in  
Bears, especially horses while T. nelson is found in the Warthogs, bush pigs Warthogs, bush pigs, T. papuae is found in the  
Wild pigs and T. pseudospiralis wild and domestic pigs (Gottstein, 2009).  
Resistance of larvae in frozen muscle  
Most of them are not resistance to the frozen muscle. Trichinella T8, T. murrelli, T.pseud ospiralis T. papuae and  
T.zimbabwensis, while others are T. spiralis resistance in horse muscles. T. native are resistance in carnivore muscles.  
Trichinella genotype T6 is resistance in carnivore muscles, T. britovi are resistance in carnivore and horse muscles and  
Trichinella genotype T12 Unknown (Gottstein, 2009). The epidemiology of trichinellosis is summarized as below in table 1.  
Disease ecology  
The usual source of trichinosis in humans is from eating pork products or meat from horses, dogs, or a variety of  
wildlife species, including wild pig, bear, walrus, and seal. Trichinella spp. is transmitted by two specific cycles, the  
domestic cycle and the sylvatic cycle (Dick and Pozio, 2001).  
Domestic cycle  
The domestic cycle is prevalent on small farms where disease control is not a primary objective in food production.  
Areas where infection is endemic are found throughout the world (Dupouy- Camet, 2000). The domestic cycle of  
transmission is primarily involves T. spiralis in a cycle of pig-to-pig transmission, and humans enter the cycle through  
eating pork. The infection can be highly pathogenic in humans. Synanthropic rats, mice, cats, dogs, and horses, as well as  
many wildlife species, also contribute to the cycle in many areas. Pigs maintain the cycle by eating pieces of infected  
meat scraps, eating infected rats or mice, biting the tails of infected pigs, cannibalizing dead pigs, ingesting feces from  
pigs that have recently eaten infected meat, or eating other species of infected mammals (Ortega-Pierres and others et  
Sylvatic cycle  
The sylvatic cycle of transmission predominantly involves predation, cannibalism, or scavenging behaviours of  
species of carnivorous wildlife. Trichinella spp. are transmitted when fresh, frozen, or decomposing carcasses or meat  
scraps are eaten (Dupouy-Camet, 2000). The species of Trichinella associated with the sylvatic cycle are T. nativa, T.  
britovi, T. murrelli, T. nelsoni, T. pseudospiralis, T. papuae and T. zimbabwensis. T. spiralis can also affect wildlife in  
temperate and tropical regions, but it does not survive in arctic and subarctic regions because larvae do not survive in a  
Clinical signs  
The severity of the clinical course depends firstly on parasitic factors, such as the species involved and the number  
of infective larvae ingested, and secondly on host factors, such as sex, age, and immune status (Bruschi and Murrell,  
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2002). The chief clinical of trichinellosis were compatible in type and frequency with the classical trichinellosis syndrome,  
i.e., myalgia, diarrhoea, fever, facial oedema and headaches that, after treatment, disappeared within 28 weeks  
(Dupouy-Camet and Bruschi, 2007). The clinical signs of acute trichinellosis are characterized by two phases: an enteral  
and a parenteral phase, corresponding to the presence of parasites in the intestine and in the circulation and/or  
musculature, respectively (Oivanen, 2005). The most common signs during the enteral phase of a mild infection are  
transient diarrhoea and nausea. However, in moderate to severe infections, the first signs are upper abdominal pain,  
diarrhoea or constipation, vomiting, malaise, and mild fever. The enteral phase lasts for six weeks (Kocięcka, 2000). From  
the second to the sixth week post infection, the enteral phase is still present, but the dominating signs arise from the  
parenteral phase due to the migrating larvae and their indiscriminate penetration of different tissues. During the third  
week post infection the symptoms intensify due to invasion of muscle cells. Characteristic signs include weakness, pain,  
paralysis, and photo phobia. Edema is prominent and patients may have shortness of breath. Endocarditis, myocarditis,  
and cardiac failure have been reported. The signs of acute illness usually diminish from the fifth or sixth week post  
Table 1 - Epidemiology of Trichinellosis  
Main source of infection  
in human  
Resistance of larvae  
in frozen muscle  
Species or genotype  
Encapsulated  
Geographical distribution  
Host range  
Domestic and sylvatic  
mammals  
Domestic and sylvatic  
swine horse  
T. spiralis  
Cosmopolitan  
Yes in horse muscle  
Arctic and subarctic areas of  
America, Asia ,Europe  
T. nativa  
Yes in carnivores  
muscle  
Sylvatic carnivores  
Sylvatic carnivores  
Bears, walruses  
Carnivores  
Canada, Alaska, rocky  
mountains, and Appalachian  
Mountains in the united states  
Temperate areas of Europe  
and Asia, northern and  
western Africa  
Yes in carnivores  
muscle  
Trichinella genotype T6  
Sylvatic mammals and  
rarely domestic pigs  
Wild boar, domestic pig  
,horse, foxes, jackal  
Yes in carnivores and  
horse muscle  
T. britovi  
Trichinella T8  
T. murrelli  
South Africa and Namibia  
Sylvatic carnivores  
Sylvatic carnivores  
None documented  
Bears, horses  
No  
No  
United states and southern  
Canada  
Trichinella genotype T9  
T. nelson  
Japan  
Sylvatic carnivores  
Sylvatic mammals  
Cougars  
None documented  
Warthogs, bush pigs  
None documented  
No  
Eastern-southern Africa  
No  
Trichinella genotype T12 Argentina  
Unknown  
Non encapsulated  
Sylvatic mammals and  
birds, domestic pig  
Wild pig , salt water  
crocodile  
T. pseudo spiralis  
T. papuae  
Cosmopolitan  
Papua new guinea ,Thailand  
Domestic and wild pigs  
Wild pig  
No  
No  
No  
Zimbabwe,Mozambique, south Nile crocodiles, monitor  
Africa,Ethiopia lizards  
T. zimbabwensis  
None documented  
Source: Gottstein (2009).  
Diagnosis  
Direct method  
Meat inspection for the detection of Trichinella larvae is designed to prevent clinical trichinellosis in humans but not  
to prevent infection. The identification of Trichinella larvae in muscle samples from pigs and other animal species  
intended for human consumption (e.g., horses, wild boars, and bears) is limited to post-mortem inspection of carcasses.  
Muscle biopsy is a traditional method applied to diagnose trichinellosis. Samples are usually taken from the M.  
deltoideus. Other possible sites are the Musculus biceps brachii, Musculus gastrocnemius, M. pectoralis, M. gluteus  
maximus, and Musculi intercostali (Gamble, 2000). Muscle biopsy is recommended only in cases where serological results  
are unclear. In autopsy, the sampling site is the diaphragm (Bruschi and Murrell, 2002). Direct detection is also applied in  
wildlife monitoring, where indicator animals (e.g., foxes or raccoon dogs) are examined to assess the prevalence of  
Trichinella infection among the wildlife reservoir and the risk of introduction into domestic animals. Methods to detect  
Trichinella larvae in muscle samples need to be highly sensitive, and performance is greatly influenced by the sample  
size, the muscle type selected for sampling, and the specific method used (Nockler, 2000). In order to identify predilection  
sites, in particular, animal species that optimal for diagnostic investigations, several experimental studies using doses  
that mimic natural infections have been performed. Thus, in domestic swine, the three main predilection sites for T.  
spiralis are the diaphragm crus, the tongue, and the masseter (Gamble, 2000), and analogous results were observed in  
experimental T. britovi and T. pseudospiralis infection in this host species (Nockler, 2005). Some of the sampling sites  
recommended by the International Commission on Trichinellosis for different domestic and wild animals subjected to  
meat examination or epidemiological studies are summarized in Table 2.  
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Table 2 - Predilection site of Trichinella larvae in different animal species  
Animal species  
Predilection site  
Aim of detection  
Meat inspection (domestic animals)  
Meat inspection (domestic animals)  
Meat inspection (game)  
Domestic pig  
Horse  
Diaphragm, Masseter, Tongue  
Tongue masseter  
Wild boar  
Bear  
Forearm, diaphragm ,tongue  
Tongue, diaphragm, masseter  
Tongue,diaphragm flipper, masseter  
Tongue, forearm, diaphragm, masseter  
Diaphragm ,forearm, muscle, tongue  
Meat inspection (game)  
Water seal  
Fox  
Meat inspection (game)  
Epidemiological studies (reservoir animal)  
Epidemiological studies (reservoir animal)  
Raccoon dog  
Source: Nockler, (2005).  
Serology  
Serology is considered to be appropriate for the surveillance and epidemiological investigations of trichinellosis in  
domestic animals and wildlife (Dworkin, 1996). The indirect serological diagnostic methods can be used at both anti-  
mortem and post-mortem examination for Trichinella-specific antibodies. Several conventional Sero diagnostic methods  
have been practiced in detecting Trichinella larvae. These include ELISA, immunofluorescence antibody test (IFAT),  
complement fixation test, and hemagglutination test and molecular technique (Oivanen, 2005).  
Enzyme Linked Immunosarbant Assay (ELISA)  
The ELISA method is relatively simple to apply, and it can be automated in Trichinella diagnostics. It is sufficiently  
sensitive to detect low-level infect ion (Nöckler et al., 2000). Traditionally ELISA has been applied to analyse antibodies in  
serum samples. According to some reports, samples of muscle juice can substitute for serum samples. This may be a  
practical solution if serum is unavailable. Results with muscle juice were hopeful in pigs but inconsistent in wild red foxes  
(Vercammen et al., 2002). However, this method cannot replace the direct methods at meat inspection because it can fail  
to detect early or very late stages of infections (Gamble et al. 2004). Infection levels as low as one larva/100 g of tissue is  
detectable by ELISA in pigs (Gamble et al., 2004). This high level of sensitivity makes serological testing by ELISA a useful  
method for detecting ongoing transmission of Trichinella infection at the farm or for more broadly based surveillance  
programmes. A disadvantage of serology for the detection of trichinellosis is the low rate of false-negative results  
observed in infected animals (OIE, 2012). For this reason, serological methods are not recommended for individual  
carcass testing. Serological responses in pigs persist for a long time after infection with no decline in titre; however,  
antibody has been reported to reject in horses within a few months following infection. The use of ELISA to detect the  
presence of parasite-specific antibodies provides a quick method that can be performed on serum, blood or tissue fluid  
collected before or after slaughter. The dilution used is different for serum than for tissue fluid (Nöckler et al., 2000).  
Antigens that are specifically secreted from the stichocytne cells of living L1 larvae and bear the TSL-1 carbohydrate  
epitope are recognised by Trichiella-infected animals. The specificity and sensitivity of ELISA is largely dependent on the  
quality of the antigen used in the test (Forbes et al., 2004; OIE, 2012).  
Molecular technique. Since there are no morphological features to specify larvae, molecular diagnosis is used to  
yield the species or genotype diagnostically recovered. For this purpose, a multiplex PCR has been developed for the  
simple and unequivocal differentiation of Trichinella species and genotypes (Zarlenga, 1999). Polymerase chain reaction  
limited studies have shown that PCR can be used to detect the nucleic acid of larvae in the musculature of infected  
animals (Zarlenga et al., 2003). However, this method lacks sensitivity and is not practical for routine testing of food  
animals. Identification of the species or genotype of Trichinella recovered from muscle tissue is useful in understanding  
the epidemiology of the parasite in animals, in assessing the relative risk of human exposure and to trace back the  
infection to the farm of origin (OIE Terrestrial Manual, 2012). Specific primers have been developed that allow the  
identification of single larva collected from muscle tissues at the species and genotype level by PCR). This multiplex PCR  
is a sensitive, inexpensive, and rapid molecular approach that can unequivocally identify a single larva at the species and  
genotype levels (Pozio et al., 2003).  
Status of Trichinellosis in Ethiopia  
At least two confirmed outbreaks of trichnellosis had been reported in Ethiopia. One of the outbreaks was reported  
in Gojjam administrative region. The outbreak was associated with ingestion of meat from a wild boar. In this outbreak,  
from 30 soldiers, 20 who ate the raw meat became ill and 5 of them were admitted to Hospital with distinctive history  
and clinical features the disease. The diagnosis was confirmed by deltoid muscle biopsy in all the 5 cases. Similar  
outbreak had been reported from Central Arsi (Kefenie et al., 1988; Kefenie and Bero, 1992).  
Public health importance of trichinella  
In humans, trichinosis is an important food-borne disease that can cause acute and chronic illness. Humans are only  
infected with Trichinella larvae through the ingestion of meat that has not been appropriately cooked. All species of  
Trichinella, except for the none encapsulated species (T. pseudospiralis, T. papuae, and T. zimbabwensis), can be highly  
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pathogenic in humans (Kociecka, 2000). T. spiralis is apparently more pathogenic in humans than other species because  
more larvae are produced by the female worms (Foreyt, 2013). Recently, T. papuae has been implicated in outbreaks of  
human trichinosis (Khumjui et al, 2008) .Clinical manifestations are often complex, and they depend on the age of the  
human host, the state of resistance, and the numbers of larvae ingested. Most clinical symptoms are present between 1  
and 6 weeks after infection and the psychological effects of affected humans advance complicate the physical symptoms  
of the disease. Three stages of disease in humans have been described: the enteral or intestinal phase, the migratory or  
mucosal invasion phase, and the parenteral or convalescence phase (Foreyt, 2013).  
Recently, T. papuae has been implicated in outbreaks of human trichinosis. Twenty-eight people in Thailand became  
sick after eating wild boar and suffered symptoms of trichinosis, and T. papuae was identified in a muscle biopsy from one  
of the patients (Khumjui et al., 2008). T. papuae was also suspected as the cause of an outbreak of trichinosis in eight  
people who had eaten raw soft-shelled turtles in Taiwan (Lo et al., 2009).  
CONCLUSION AND RECOMMENDATION  
Trichinellosis (trichinosis) is caused by nematodes (roundworms) of the genus Trichinella. The disease has a significant  
public health importance. All mammals are susceptible to infection, but the number of larvae required for infection varies  
according to the genetic constitute of the parasite and the host species. Trichinellosis is acquired by eating raw or  
undercooked meat that contains Trichinella larvae. Domestic animals can be infected by the consumption of infected raw  
tissues. Trichinella has a direct life cycle, which means it completes all stages of maturity in one host. Transmission from  
one host to another host can only occur by ingestion of muscle tissue which is infected with the encysted larval stage of  
the parasite. When ingested, muscle larvae excyst and enter tissues of the small intestine, where they undergo  
development to the adult stage. Male and female adult parasites mate and produce newborn larvae which leave the  
intestine and migrate, through the circulatory system, to striated muscle tissue. The severity of human trichinellosis is  
dependent upon the number of infected larvae ingested, the species of Trichinella, and the immune status of the human  
host. Muscle biopsy, ELISA and PCR method is important tool for diagnosis of infection.  
Based on above conclusion the following recommendations are forwarded: 1) Education of the consumer about the  
risk of consumption of raw or undercooked meat and meat products from both domestic and wild pigs should be  
emphasized; 2) Strict quarantine should be exercised to control the slaughter and meat distribution of potentially infected  
animals.  
DECLARATIONS  
Corresponding Author  
Consent to publish  
Not applicable.  
Competing interests  
The authors declare that they have no competing interests.  
Authors' contributions  
M. Yayeh and M. Birhan conceived the study, coordinated the overall activity, and carried out the statistical analysis,  
drafted the manuscript. M. Birhan participated in drafting and reviewing the manuscript. M. Yayeh and M. Birhan  
conceived the study, coordinated the overall activity, and reviewed the manuscript. M. Yayeh and M. Birhan participated in  
drafting and reviewing the manuscript. All authors read and approved the final manuscript.  
Availability of data and materials  
Data will be made available up on request of the primary author.  
Acknowledgment  
The authors’ heartfelt thanks University of Gondar, Research and Community Service V/ President Office for finance  
and resource supporting.  
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